Platform
RNA is accelerating.
Manufacturing isn’t – until now.
EnPlusOne has developed a fundamentally new way to make RNA—using enzymes in water—built for large patient populations and next-generation therapeutics.
Inputs
Universal Enzyme
NTP Building Blocks
Platform
Outputs
Full Length Product
Blockmers + Ligation
Custom RNA
Applications
siRNA
ASO
gRNA
mRNA
Emerging modalities & next-generation modifications
A single, versatile enzyme that works across natural bases, established modifications, and 50+ novel chemistries
Universal Enzyme
Unprotected nucleotide inputs that improve atom economy and maximize final RNA output
NTP Building Blocks
An enzymatic platform that harnesses nature’s catalyst to create better RNA — pure, scalable, and endlessly customizable
ezRNA™
Template-free synthesis to install site-specific modifications
Full Length Product
Ligation of blockmers enables a fully enzymatic synthesis for any length RNA
Blockmers + Ligation
Modality-agnostic enhancements for any existing designs
Hybrid Synthesis
Modifications
In addition to known therapeutically relevant modifications, we’ve incorporated numerous others, including novel and natural, spanning the stability, conjugation and delivery space
Base
Standard and non-standard nucleobases cab be incorporated with the ezRNA™ platform. Bases with fluorophores, alkyl groups, halogens, conjugation handles, and more are also compatible
2’ Sugar
Essential for siRNA therapeutics, the platform can incorporate all standard 2’ modifications including 2’OH, 2’H, 2’OMe and 2’F. Other halogens, locked nucleic acids, and more are also tolerated on the 2’
3’ Sugar
The flexibility of the platform provides access to 3′- modifications including dideoxy, click handles, and chain terminators.
Backbone
The ezRNA™ platform can install both standard, phosphorothioated, boronated backbones and more
We work with partners
Across discovery and CMC to deliver RNA at the scale and quality required for advanced therapeutics